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Measuring Nonspecific Protein–Protein Interactions by Dynamic Light Scattering

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Protein Self-Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2039))

Abstract

Dynamic light scattering has become a method of choice for measuring and quantifying weak, nonspecific protein–protein interactions due to its ease of use, minimal sample consumption, and amenability to high-throughput screening via plate readers. A procedure is given on how to prepare protein samples, carry out measurements by commonly used experimental setups including flow through systems, plate readers, and cuvettes, and analyze the correlation functions to obtain diffusion coefficient data. The chapter concludes by a theoretical section that derives and rationalizes the correlation between diffusion coefficient measurements and protein–protein interactions.

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Authors and Affiliations

  1. School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, UK

    Daniel Corbett, Jordan W. Bye & Robin A. Curtis

Authors
  1. Daniel Corbett
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  2. Jordan W. Bye
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  3. Robin A. Curtis
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Corresponding author

Correspondence to Robin A. Curtis .

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Editors and Affiliations

  1. Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland

    Jennifer J. McManus

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Corbett, D., Bye, J.W., Curtis, R.A. (2019). Measuring Nonspecific Protein–Protein Interactions by Dynamic Light Scattering. In: McManus, J. (eds) Protein Self-Assembly. Methods in Molecular Biology, vol 2039. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9678-0_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9678-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9677-3

  • Online ISBN: 978-1-4939-9678-0

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