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Oligonucleotide-Based Therapies pp 53–72Cite as

Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blot

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2036))

Abstract

The discovery of the RNA interference (RNAi) pathway followed by the usage of synthetic short-interfering RNAs (siRNA) has contributed greatly to the understanding of gene function. Carefully designed siRNAs can considerably improve siRNA specificity leading to more accurate and efficient gene silencing. Evaluation of gene knockdown is vital for optimization of siRNA efficacy. Here we describe the fundamental principles of siRNA design and strategies for evaluating gene knockdown.

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Acknowledgments

This study was supported by the Swedish Foundation for Strategic Research (C.P.A.) and the Swedish Childhood Cancer Foundation (C.P.A.).

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Authors and Affiliations

  1. Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, Huddinge, Sweden

    Linda Vidarsdottir, Oksana Goroshchuk, Iryna Kolosenko & Caroline Palm-Apergi

Authors
  1. Linda Vidarsdottir
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  2. Oksana Goroshchuk
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  3. Iryna Kolosenko
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  4. Caroline Palm-Apergi
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Corresponding author

Correspondence to Caroline Palm-Apergi .

Editor information

Editors and Affiliations

  1. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Olof Gissberg

  2. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Rula Zain

  3. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Karin E. Lundin

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Vidarsdottir, L., Goroshchuk, O., Kolosenko, I., Palm-Apergi, C. (2019). Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blot. In: Gissberg, O., Zain, R., Lundin, K. (eds) Oligonucleotide-Based Therapies. Methods in Molecular Biology, vol 2036. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9670-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9670-4_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9669-8

  • Online ISBN: 978-1-4939-9670-4

  • eBook Packages: Springer Protocols

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