Abstract
It has been shown that the complement cascade is involved in the process of mobilization of hematopoietic stem cells, from their niche in the bone marrow to the peripheral blood. Based on this knowledge modulation of complement, cascade activation may enable the development of better mobilization strategies for poorly mobilizing patients. Herein we present a mobilization protocol in mice model, useful for studying the effect of the complement activation in the mobilization process.
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References
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Acknowledgments
We thank Mateusz Adamiak (Department of Regenerative Medicine and Center for Preclinical Studies and Technology, Warsaw Medical University, Warsaw, Poland) for his helpful suggestions and critical reading of the manuscript and Kamila Bujko (Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA) for her kind providing of figure representing flow cytometry analysis. We thank Tomasz Wilanowski (Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland) for reviewing the protocol as a native speaker.
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Grabowska, A.I., Hawryluk, J.M. (2019). Analysis of the Complement Cascade Activation During Mobilization of Hematopoietic Stem/Progenitor Cells. In: Klein, G., Wuchter, P. (eds) Stem Cell Mobilization. Methods in Molecular Biology, vol 2017. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9574-5_13
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DOI: https://doi.org/10.1007/978-1-4939-9574-5_13
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Publisher Name: Humana, New York, NY
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