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Methods for Transposon Mutagenesis in Proteus mirabilis

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Microbial Transposon Mutagenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2016))

Abstract

Several methods for transposon mutagenesis have been employed for use in P. mirabilis. The first method involves the use of mini-Tn5 derivatives, which are delivered by conjugation of a suicide plasmid containing this transposon, followed by transposition into the chromosome. A second method is the use of preformed transposon/transposase complexes (transposomes), which are introduced into P. mirabilis cells by electroporation. Each of these methods will be discussed along with the advantages and disadvantages of each.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA

    Philip N. Rather

  2. Research Service, Atlanta VA Medical Center, Decatur, GA, USA

    Philip N. Rather

Authors
  1. Philip N. Rather
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Correspondence to Philip N. Rather .

Editor information

Editors and Affiliations

  1. Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR, USA

    Steven C. Ricke

  2. Department of Food Science and Technology, Oregon State University, Corvallis, OR, USA

    Si Hong Park

  3. Department of Food Science, University of Arkansas, Fayetteville, AR, USA

    Morgan L. Davis

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Rather, P.N. (2019). Methods for Transposon Mutagenesis in Proteus mirabilis . In: Ricke, S., Park, S., Davis, M. (eds) Microbial Transposon Mutagenesis. Methods in Molecular Biology, vol 2016. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9570-7_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9570-7_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9569-1

  • Online ISBN: 978-1-4939-9570-7

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