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Microbial Transposon Mutagenesis pp 51–62Cite as

Implementation of Transposon Mutagenesis in Bifidobacterium

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2016))

Abstract

Random transposon mutagenesis allows for relatively rapid, genome-wide surveys to detect genes involved in functional traits, by performing screens of mutant libraries. This approach has been widely applied to identify genes responsible for activities of interest in multiple eukaryote and prokaryote organisms, although most studies on microorganisms have focused on pathogenic and clinically relevant bacteria. In this chapter we describe the implementation of an in vitro Tn5-based transposome strategy to generate a large collection of random mutants in the gut commensal Bifidobacterium breve UCC2003, and discuss considerations when applying this mutagenesis system to other Bifidobacterium species or strains of interest.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Biochemistry of Dairy Products, IPLA-CSIC, Villaviciosa, Asturias, Spain

    Lorena Ruiz

  2. APC Microbiome Ireland, University College Cork, Cork, Ireland

    Douwe van Sinderen

  3. School of Microbiology, University College Cork, Cork, Ireland

    Douwe van Sinderen

Authors
  1. Lorena Ruiz
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  2. Douwe van Sinderen
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Corresponding author

Correspondence to Douwe van Sinderen .

Editor information

Editors and Affiliations

  1. Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR, USA

    Steven C. Ricke

  2. Department of Food Science and Technology, Oregon State University, Corvallis, OR, USA

    Si Hong Park

  3. Department of Food Science, University of Arkansas, Fayetteville, AR, USA

    Morgan L. Davis

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Ruiz, L., van Sinderen, D. (2019). Implementation of Transposon Mutagenesis in Bifidobacterium . In: Ricke, S., Park, S., Davis, M. (eds) Microbial Transposon Mutagenesis. Methods in Molecular Biology, vol 2016. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9570-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9570-7_5

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9569-1

  • Online ISBN: 978-1-4939-9570-7

  • eBook Packages: Springer Protocols

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