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Construction of DNA Barcode-Tagged Salmonella Strains

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Microbial Transposon Mutagenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2016))

Abstract

This chapter provides a detailed protocol for construction of DNA barcode-tagged isogenic strains of Salmonella. The protocol is illustrated with S. Enteritidis in this chapter. However, this protocol should be widely applicable to other Salmonella serotypes. A series of the DNA barcode-tagged strains thus constructed can be used in combination with next generation sequencing or quantitative PCR to study the population dynamics of the bacterial pathogen during infection within the host or transmission within a population of the host in a quantitative manner.

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References

  1. Varble A, Albrecht RA, Backes S, Crumiller M, Bouvier NM, Sachs D et al (2014) Influenza a virus transmission bottlenecks are defined by infection route and recipient host. Cell Host Microbe 16:691–700. https://doi.org/10.1016/j.chom.2014.09.020

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Lam LH, Monack DM (2014) Intraspecies competition for niches in the distal gut dictate transmission during persistent Salmonella infection. PLoS Pathog 10:e1004527. https://doi.org/10.1371/journal.ppat.1004527

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Zhang T, Abel S, Abel Zur Wiesch P, Sasabe J, Davis BM, Higgins DE et al (2017) Deciphering the landscape of host barriers to Listeria monocytogenes infection. Proc Natl Acad Sci U S A 114:6334–6339. https://doi.org/10.1073/pnas.1702077114

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Yang Y, Ricke SC, Tellz G, Kwon YM (2017) Quantitative tracking of Salmonella Enteritidis transmission routes using barcode-tagged isogenic strains in chickens: proof-of-concept study. Front Vet Sci 4:15. https://doi.org/10.3389/fvets.2017.00015

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Yang Y, Tellez G, Latorre JD, Ray PM, Hernandez X, Hargis BM, Ricke SC, Kwon YM (2018) Salmonella excludes Salmonella in poultry: confirming an old paradigm using conventional and barcode-tagging approaches. Front Vet Sci 5:101. https://doi.org/10.3389/fvets.2018.00101

    Article  PubMed  PubMed Central  Google Scholar 

  6. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97:6640–6645. https://doi.org/10.1073/pnas.120163297

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Frost MR, Guggenheim JA (1999) Prevention of depurination during elution facilitates the reamplification of DNA from differential display gels. Nucleic Acids Res 27:e6

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA

    Yichao Yang, Reena Chandrashekar & Young Min Kwon

  2. Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR, USA

    Steven C. Ricke

  3. Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, USA

    Steven C. Ricke & Young Min Kwon

Authors
  1. Yichao Yang
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  2. Reena Chandrashekar
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  3. Steven C. Ricke
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  4. Young Min Kwon
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Corresponding author

Correspondence to Young Min Kwon .

Editor information

Editors and Affiliations

  1. Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR, USA

    Steven C. Ricke

  2. Department of Food Science and Technology, Oregon State University, Corvallis, OR, USA

    Si Hong Park

  3. Department of Food Science, University of Arkansas, Fayetteville, AR, USA

    Morgan L. Davis

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Yang, Y., Chandrashekar, R., Ricke, S.C., Kwon, Y.M. (2019). Construction of DNA Barcode-Tagged Salmonella Strains. In: Ricke, S., Park, S., Davis, M. (eds) Microbial Transposon Mutagenesis. Methods in Molecular Biology, vol 2016. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9570-7_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9570-7_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9569-1

  • Online ISBN: 978-1-4939-9570-7

  • eBook Packages: Springer Protocols

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