Abstract
The difference in time lags between a labeling pulse of 13CO2 of the foliage and the appearance of labeled C in the respiration at different locations along the stem of a tall plant is used to estimate at which velocities the isotope tracer, i.e., the labeled carbohydrates, are transported in the phloem sap. Here we describe a method for pulse-labeling tall plants in the field and subsequently tracing 13C in the respiratory efflux of CO2.
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References
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Acknowledgments
We thank the many colleagues from Brazil, France, Japan, and Thailand who contributed to the development and improvement of this method.
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Epron, D., Dannoura, M., Plain, C. (2019). Using 13C to Quantify Phloem Transport on Tall Plants in the Field. In: Liesche, J. (eds) Phloem. Methods in Molecular Biology, vol 2014. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9562-2_12
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DOI: https://doi.org/10.1007/978-1-4939-9562-2_12
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9561-5
Online ISBN: 978-1-4939-9562-2
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