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Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection

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Neisseria gonorrhoeae

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1997))

Abstract

Macrophages are critical cells in the innate immune response to microorganisms sensed in the tissues. During infections, the interaction between pathogens and macrophages leads to a macrophage response that includes cytokine production, antigen processing and presentation in the context of MHC molecules, expression of T cell costimulatory molecules and recruitment of innate defense effectors, which results in clearance of infection. However, Neisseria gonorrhoeae can suppress the protective immune response at this level, avoiding its detection and elimination. Studies addressed to develop the interactions between macrophages and Neisseria gonorrhoeae allow us to find potential targets to be exploited with vaccines and therapeutic drugs. In this chapter, we describe protocols to generate human monocyte-derived macrophages and assess their response to infection with Neisseria gonorrhoeae.

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Acknowledgments

This work was supported by FONDECYT regular 1180666 (J.G., P.I.R., and A.E.). PCI-CONICYT ‘Apoyo a la Formación de Redes Internacionales Para Investigadores en Etapa Inicial’ REDI170370 (P.I.R., A.E.).

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Authors and Affiliations

  1. Instituto de Investigación en Ciencias Odontológicas, Facultad de Odontología, Universidad de Chile, Santiago, Chile

    Jimena Gatica

  2. Laboratory of Medical Microbiology and Pathogenesis, Faculty of Medicine, Universidad Andres Bello, Concepción, Región del Bío-Bío, Chile

    Paula I. Rodas

  3. Laboratorio Biología celular y molecular, Instituto de Ciencias Odontológicas, Facultad de Odontología, Universidad de Chile, Santiago, Región Metropolitana, Chile

    Alejandro Escobar

Authors
  1. Jimena Gatica
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  2. Paula I. Rodas
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  3. Alejandro Escobar
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Editor information

Editors and Affiliations

  1. Molecular Microbiology Group, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK

    Myron Christodoulides

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Gatica, J., Rodas, P.I., Escobar, A. (2019). Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection. In: Christodoulides, M. (eds) Neisseria gonorrhoeae. Methods in Molecular Biology, vol 1997. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9496-0_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9496-0_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9495-3

  • Online ISBN: 978-1-4939-9496-0

  • eBook Packages: Springer Protocols

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