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Neural Crest Cells pp 207–221Cite as

Avenues for Investigating the Neural Crest and Its Derivatives in Non-model (Unconventional) Vertebrates: A Craniofacial Skeleton Perspective

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1976))

Abstract

One of the early, profound insights regarding the biology of the neural crest was the observation of its contribution to the skeletal structures of the cranium and jaws. The critical nature of these structures made the comparative analysis of the cranial neural crest and its derived structures essential investigative aims toward our understanding of the development and evolution of vertebrates and vertebrate-specific structures. Though classically applied to a relatively wide range of taxa in the nineteenth and early twentieth centuries, the application of traditional methodologies for complex comparative developmental and anatomical analyses subsequently become more limited by their time-consuming nature, resource scarcity, and a greater emphasis on the genetic and molecular regulation of patterning and morphogenesis in a select number of tractable model organisms. Recently, however, this trend has been reversed, and the value of genetic and molecular-based questions applied to non-model (unconventional) vertebrate organisms has been re-appreciated. This is particularly true of comparative investigations of cranial neural crest biology. Herein, we present methodologies for the analysis of the cranial neural crest and its structural derivatives employable in modern investigations of both model and unconventional vertebrate organisms.

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Author information

Authors and Affiliations

  1. Center for Anatomy, Institute for Cell and Neurobiology, Charité Universitätsmedizin Berlin, Berlin, Germany

    Michael J. Depew

  2. Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria-CSIC-SODERCAN, Santander, Spain

    Federica Bertocchini

Authors
  1. Michael J. Depew
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  2. Federica Bertocchini
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Corresponding author

Correspondence to Michael J. Depew .

Editor information

Editors and Affiliations

  1. Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, SA, Australia

    Quenten Schwarz

  2. Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, SA, Australia

    Sophie Wiszniak

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Depew, M.J., Bertocchini, F. (2019). Avenues for Investigating the Neural Crest and Its Derivatives in Non-model (Unconventional) Vertebrates: A Craniofacial Skeleton Perspective. In: Schwarz, Q., Wiszniak, S. (eds) Neural Crest Cells. Methods in Molecular Biology, vol 1976. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9412-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9412-0_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9411-3

  • Online ISBN: 978-1-4939-9412-0

  • eBook Packages: Springer Protocols

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