Abstract
Examining transcriptomics of populations at the single-cell level allows for higher resolution when studying functionality in development, differentiation, and physiology. Real-time quantitative PCR (qPCR) enables a sensitive detection of specific gene expression; however, processing a large number of samples for single-cell research involves a time-consuming process and high reagent costs. Here we describe a protocol for single-cell qPCR using nanofluidic chips. This method reduces the number of handling steps and volumes per reaction, allowing for more samples and genes to be measured.
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Acknowledgments
This work was supported by EMBO (award number ALTF 698-2012), Directorate-General for Research and Innovation (FP7-PEOPLE-2010-IEF, ThPLAST 274192) and an EMBL Interdisciplinary Postdoctoral fellowship, supported by H2020 Marie Skłodowska-Curie Actions.
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Haim-Vilmovsky, L. (2019). High-Throughput Single-Cell Real-Time Quantitative PCR Analysis. In: Proserpio, V. (eds) Single Cell Methods. Methods in Molecular Biology, vol 1979. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9240-9_11
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DOI: https://doi.org/10.1007/978-1-4939-9240-9_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9239-3
Online ISBN: 978-1-4939-9240-9
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