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Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model

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Developmental Toxicology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1965))

Abstract

Cranial neural crest cells (NCCs) migrate to the branchial arches and give rise to the majority of cranial mesenchyme that eventually differentiates into odontoblasts, cartilage, craniofacial bone, and connective tissue; a subset of these cells differentiate into cranial ganglia. Here we present a protocol that describes directed differentiation method of human pluripotent stem cells into cranial NCC-like cells and a cytotoxicity assay using hPSC-derived cranial NCC-like cells. This cell-based assay system allows for high-sensitive cytotoxicity detection of test chemicals. These methods can be applied to predict drug/chemical toxicity effect on early craniofacial development.

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Acknowledgments

This work was supported by JSPS KAKENHI Grant Numbers 16H05535 and 00340448 to M.S. and M.K.F. and Research Project for Practical Applications of Regenerative Medicine from the Ministry of Health, Labor and Welfare of Japan and the Japan Agency for Medical Research and Development (AMED) to M.K.F. Authors thank Yu Jung Liu, Takako Nakano, and Naoko Ueda for their excellent technical supports.

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Authors and Affiliations

  1. Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Ibaraki, Japan

    Mika Suga & Miho K. Furue

Authors
  1. Mika Suga
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  2. Miho K. Furue
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Editor information

Editors and Affiliations

  1. Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA

    Jason M. Hansen

  2. Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, Canada

    Louise M. Winn

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Suga, M., Furue, M.K. (2019). Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model. In: Hansen, J., Winn, L. (eds) Developmental Toxicology. Methods in Molecular Biology, vol 1965. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9182-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9182-2_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9181-5

  • Online ISBN: 978-1-4939-9182-2

  • eBook Packages: Springer Protocols

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