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Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry

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Microcalorimetry of Biological Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1964))

Abstract

This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔrH), Km, kcat, and vmax are determined in a single assay that does not require labeling or immobilization.

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References

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Author information

Authors and Affiliations

  1. Applications Lab, TA Instruments, Lindon, UT, USA

    Colette F. Quinn

  2. Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA

    Lee D. Hansen

Authors
  1. Colette F. Quinn
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  2. Lee D. Hansen
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Corresponding author

Correspondence to Colette F. Quinn .

Editor information

Editors and Affiliations

  1. Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, CNRS, Strasbourg, France

    Eric Ennifar

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Quinn, C.F., Hansen, L.D. (2019). Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry. In: Ennifar, E. (eds) Microcalorimetry of Biological Molecules. Methods in Molecular Biology, vol 1964. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9179-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9179-2_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9178-5

  • Online ISBN: 978-1-4939-9179-2

  • eBook Packages: Springer Protocols

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