Abstract
Sample preparation is a key step in proteomics workflows. Tube-gel (TG) is a fast and repeatable sample preparation method that consists in the instantaneous trapping of the sample in a polyacrylamide gel matrix. It takes advantage of in-gel sample preparations by allowing the use of high concentrations of sodium-dodecyl sulfate but avoids the time-consuming step of electrophoresis. Therefore, TG limits the sample handling and is thus particularly suitable for high-throughput quantitative proteomics when large sample numbers have to be processed, as it is often the case in biomarker research and clinical proteomics projects.
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Acknowledgments
This work was supported by the French Proteomic Infrastructure (ProFI; ANR-10-INBS-08-03).
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Muller, L., Fornecker, L., Cianferani, S., Carapito, C. (2019). Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics. In: Brun, V., Couté, Y. (eds) Proteomics for Biomarker Discovery. Methods in Molecular Biology, vol 1959. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9164-8_8
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DOI: https://doi.org/10.1007/978-1-4939-9164-8_8
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