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RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria

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Bacterial Polysaccharides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1954))

Abstract

RecET direct cloning enables obtaining a large DNA region from genome. Here we describe its applications in cloning of polysaccharide gene cluster from gram-negative bacteria. Rapid and exact cloning of polysaccharide gene cluster can be achieved by this method.

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References

  1. Wang HL, Li Z, Jia RN et al (2016) RecET direct cloning and red alpha beta recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression. Nat Protoc 11(7):1175–1190

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Acknowledgments

This work was supported by NIH grant R01AI083754. Thank Prof. Zhang Y and Dr. Wang H for kindly providing p15A-Cm and E. coli GBdir-gyrA462.

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Authors and Affiliations

  1. Department of Chemistry, Georgia State University, Atlanta, GA, USA

    Zhongrui Ma & Peng George Wang

Authors
  1. Zhongrui Ma
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  2. Peng George Wang
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Corresponding author

Correspondence to Peng George Wang .

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Editors and Affiliations

  1. Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, Canada

    Inka Brockhausen

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Ma, Z., Wang, P.G. (2019). RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria. In: Brockhausen, I. (eds) Bacterial Polysaccharides. Methods in Molecular Biology, vol 1954. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9154-9_2

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  • DOI: https://doi.org/10.1007/978-1-4939-9154-9_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9153-2

  • Online ISBN: 978-1-4939-9154-9

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