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Glutamate Receptors pp 139–154Cite as

Gene Expression Analysis by Multiplex Single-Cell RT-PCR

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1941))

Abstract

Brain circuit assemblies comprise different cellular subpopulations that exhibit morphological, electrophysiological, and molecular diversity. Here we describe a protocol which, combined with whole-cell patch-clamp recording and morphological reconstruction, allows the transcriptomic analysis of the recorded cell. This protocol provides recipes on how to detect simultaneously the expression of 24 genes/markers at the single-cell level using polymerase chain reaction (PCR), how to design gene-specific probes, and how to validate them. This technique provides multiplexed expression data that cannot be easily obtained by other approaches such as immunological co-labeling.

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Authors and Affiliations

  1. Sorbonne Université, UPMC Univ Paris 06 UM119, Centre National de la Recherche Scientifique (CNRS) UMR8246, Institut National de la Santé et de la Recherche Médicale (INSERM) UMRS1130, Neuroscience Paris Seine,Institut de Biologie Paris-Seine, Paris, France

    Ludovic Tricoire, Bruno Cauli & Bertrand Lambolez

Authors
  1. Ludovic Tricoire
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  2. Bruno Cauli
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  3. Bertrand Lambolez
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Corresponding author

Correspondence to Bertrand Lambolez .

Editor information

Editors and Affiliations

  1. Department of Neurology, University of Wisconsin, Madison, WI, USA

    Corinna Burger

  2. ANSER, Homeland Security Studies and Analysis Institute, Falls Church, VA, USA

    Margaret Jo Velardo

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Tricoire, L., Cauli, B., Lambolez, B. (2019). Gene Expression Analysis by Multiplex Single-Cell RT-PCR. In: Burger, C., Velardo, M. (eds) Glutamate Receptors. Methods in Molecular Biology, vol 1941. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9077-1_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9077-1_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9076-4

  • Online ISBN: 978-1-4939-9077-1

  • eBook Packages: Springer Protocols

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