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Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice

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Viral Vectors for Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1937))

Abstract

Delivery of viral vectors directly into the central nervous system (CNS) has emerged as an important tool for the refinement of gene therapy. Intrathecal delivery by direct lumbar puncture in conscious rodents offers a minimally invasive approach that avoids tissue damage and/or destruction. Here we describe delivery of small quantities of viral vector product to the intrathecal space of rodents via direct lumbar puncture aided by a catheter.

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Acknowledgments

This work was supported by NIDA grant R01DA035931-01, DoD grant W81XWH-15-1-0494, T32 training grant T32DA07234 to K.P., and T32 training grant T32DA007097 to C.P.

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Authors and Affiliations

  1. Department of Pharmaceutics, University of Minnesota, Minneapolis, MN, USA

    Kelsey R. Pflepsen & Carolyn A. Fairbanks

  2. Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA

    Cristina D. Peterson, Kelley F. Kitto, Lucy Vulchanova, George L. Wilcox & Carolyn A. Fairbanks

  3. Department of Pharmacology, University of Minnesota, Minneapolis, MN, USA

    George L. Wilcox & Carolyn A. Fairbanks

  4. Department of Dermatology, University of Minnesota, Minneapolis, MN, USA

    George L. Wilcox

Authors
  1. Kelsey R. Pflepsen
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  2. Cristina D. Peterson
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  3. Kelley F. Kitto
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  4. Lucy Vulchanova
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  5. George L. Wilcox
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  6. Carolyn A. Fairbanks
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Corresponding author

Correspondence to Carolyn A. Fairbanks .

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Editors and Affiliations

  1. Translational Science and Molecular Medicine, Michigan State University, Grand Rapids, MI, USA

    Fredric P. Manfredsson

  2. Translational Science and Molecular Medicine, Michigan State University, Grand Rapids, MI, USA

    Matthew J. Benskey

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Pflepsen, K.R., Peterson, C.D., Kitto, K.F., Vulchanova, L., Wilcox, G.L., Fairbanks, C.A. (2019). Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice. In: Manfredsson, F., Benskey, M. (eds) Viral Vectors for Gene Therapy. Methods in Molecular Biology, vol 1937. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9065-8_20

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  • DOI: https://doi.org/10.1007/978-1-4939-9065-8_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9064-1

  • Online ISBN: 978-1-4939-9065-8

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