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Legionella pp 429–443Cite as

Analysis of the Pulmonary Microbiome Composition of Legionella pneumophila-Infected Patients

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1921))

Abstract

The analysis of the lung microbiome composition is a field of research that recently emerged. It gained great interest in pulmonary diseases such as pneumonia since the microbiome seems to be involved in host immune responses, inflammation, and protection against pathogens. Thus, it is possible that the microbial communities living in the lungs play a role in the outcome and severity of lung infections such as Legionella-caused pneumonia and in the response to antibiotic therapy. In this chapter, all steps necessary for the characterization of the bacterial and fungal fraction of the lung microbiome using high-throughput sequencing approaches are explained, starting from the selection of clinical samples to the analysis of the taxonomic composition, diversity, and ecology of the microbiome.

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Acknowledgments

Work in the CB laboratory is financed by the Institut Pasteur and AECP was fianced by a fellowship from grant ANR-10-LABX-62-IBEID and grant ANR 15 CE17 0014 03.

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Authors and Affiliations

  1. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Ana Elena Pérez-Cobas & Carmen Buchrieser

  2. CNRS UMR 3525, Paris, France

    Ana Elena Pérez-Cobas & Carmen Buchrieser

Authors
  1. Ana Elena Pérez-Cobas
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  2. Carmen Buchrieser
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Corresponding author

Correspondence to Carmen Buchrieser .

Editor information

Editors and Affiliations

  1. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Carmen Buchrieser

  2. Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

    Hubert Hilbi

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Pérez-Cobas, A.E., Buchrieser, C. (2019). Analysis of the Pulmonary Microbiome Composition of Legionella pneumophila-Infected Patients. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 1921. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9048-1_27

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  • DOI: https://doi.org/10.1007/978-1-4939-9048-1_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9047-4

  • Online ISBN: 978-1-4939-9048-1

  • eBook Packages: Springer Protocols

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