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Mitochondrial Dynamics and Activity in Legionella-Infected Cells

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1921))

Abstract

The study of Legionella pneumophila interactions with host mitochondria during infection has been historically limited by the techniques available to analyze and quantify mitochondrial dynamics and activity in living cells. Recently, new, powerful techniques such as high-content microscopy or mitochondrial respiration assays (Seahorse) have been developed to quantitatively analyze mitochondrial parameters. Here we present state-of-the-art methods adapted to analyze mitochondrial dynamics and activity during Legionella infection of living human primary macrophages.

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Acknowledgments

Work in C.B. laboratory is financed by Institut Pasteur and ANR (grant no. ANR-10-LABX-62-IBEID). P.E. is funded by the Fondation pour la Recherche Médicale (FRM) project DEQ20120323697. P.B. and O.R.S. received financial support from the European Community (ERC-STG INTRACELLTB no. 260901, MM4TB no. 260872), the ANR (grant no. ANR-10-EQPX-04-01), and the Région Nord Pas de Calais (convention no. 12000080). We thank Nathalie Aulner and the Imagopole-CiTech (part of France-BioImaging supported by ANR grant no. ANR-10-INSB-04-01, Conseil de la Region Ile-de-France, FRM), and the Technology Core of the Center for Translational Science (CRT) at Institut Pasteur, for technical support during the setup of the methods presented here.

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Authors and Affiliations

  1. University Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 8204-CIIL-Center for Infection and Immunity of Lille, Lille, France

    Ok-Ryul Song & Priscille Brodin

  2. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Carmen Buchrieser

  3. CNRS UMR 3525, Paris, France

    Carmen Buchrieser

  4. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Pedro Escoll

  5. CNRS UMR 3525, Paris, France

    Pedro Escoll

Authors
  1. Ok-Ryul Song
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  2. Priscille Brodin
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  3. Carmen Buchrieser
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  4. Pedro Escoll
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Corresponding authors

Correspondence to Carmen Buchrieser or Pedro Escoll .

Editor information

Editors and Affiliations

  1. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Carmen Buchrieser

  2. Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

    Hubert Hilbi

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Song, OR., Brodin, P., Buchrieser, C., Escoll, P. (2019). Mitochondrial Dynamics and Activity in Legionella-Infected Cells. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 1921. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9048-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9048-1_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9047-4

  • Online ISBN: 978-1-4939-9048-1

  • eBook Packages: Springer Protocols

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