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Analysis of Iron Requirements and Siderophore Production

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1921))

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Abstract

This chapter describes the methods for inducing, detecting, and purifying the Legionella pneumophila siderophore. The first protocol details the methods by which L. pneumophila is cultured to facilitate production of the siderophore, rhizoferrin. This chapter then describes how to purify siderophore from culture supernatants through sequential reversed-phase/weak-anion exchange chromatography and high-performance liquid chromatography. The next section describes assays which allow the detection of the iron-binding capability and the biological activity of the purified siderophore. Lastly, this chapter describes the growth of L. pneumophila in chemically defined liquid medium (CDM) containing various iron sources as a method to assess the iron requirements of L. pneumophila.

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Acknowledgments

This work was supported by NIH grant AI034937 awarded to NPC.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL, USA

    Denise M. Burnside & Nicholas P. Cianciotto

Authors
  1. Denise M. Burnside
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  2. Nicholas P. Cianciotto
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Corresponding author

Correspondence to Nicholas P. Cianciotto .

Editor information

Editors and Affiliations

  1. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Carmen Buchrieser

  2. Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

    Hubert Hilbi

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Burnside, D.M., Cianciotto, N.P. (2019). Analysis of Iron Requirements and Siderophore Production. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 1921. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9048-1_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9048-1_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9047-4

  • Online ISBN: 978-1-4939-9048-1

  • eBook Packages: Springer Protocols

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