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Plant Long Non-Coding RNAs pp 131–147Cite as

Purification and Functional Analysis of Plant Long Noncoding RNAs (lncRNA)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1933))

Abstract

More than 70% of eukaryotic genomes are transcribed into RNA transcripts, the majority of these transcripts are noncoding protein, and their biological functions are largely unknown. Over the last decade, the application of high-throughput sequencing technologies has led to the description of almost all cellular coding and noncoding RNA transcripts except perhaps for those transcripts that are lowly abundant or those present only in specific cells that are underrepresented in sampled tissue(s). An often underrepresented class of noncoding are long noncoding RNAs (lncRNAs), and these often play key regulatory functions for many biological processes such as cell identity and cell division. However, the purification and functional characterization in vitro are still a challenge in both animal and plant experimental systems. Here, we describe in detail methodology for purification of specific cell types, bioinformatic annotation of lncRNAs, and investigation of biological function using the reference plant Arabidopsis thaliana.

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Acknowledgment

This work was supported by an Australian Research Council Future Fellowship (FT130100525) awarded to IS and a MOET-VIED Ph.D. scholarship awarded to T.D.

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Authors and Affiliations

  1. Department of Molecular and Biomedical Sciences, School of Biological Sciences, The University of Adelaide, Adelaide, SA, Australia

    Trung Do, Zhipeng Qu & Iain Searle

Authors
  1. Trung Do
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  2. Zhipeng Qu
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  3. Iain Searle
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Corresponding author

Correspondence to Iain Searle .

Editor information

Editors and Affiliations

  1. Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, Guangxi, China

    Julia A. Chekanova  & Hsiao-Lin V. Wang  & 

  2. Department of Biology, Emory University, Atlanta, Georgia, USA

    Hsiao-Lin V. Wang

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Do, T., Qu, Z., Searle, I. (2019). Purification and Functional Analysis of Plant Long Noncoding RNAs (lncRNA). In: Chekanova, J.A., Wang, HL.V. (eds) Plant Long Non-Coding RNAs. Methods in Molecular Biology, vol 1933. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9045-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9045-0_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9044-3

  • Online ISBN: 978-1-4939-9045-0

  • eBook Packages: Springer Protocols

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