Abstract
Transcriptome-wide mapping RNA modification is crucial to understand the distribution and function of RNA modifications. Here, we describe a protocol to transcriptome-wide mapping 5-methylcytosine (m5C) in plant, by a RNA immunoprecipitation followed by deep sequencing (m5C-RIP-seq) approach. The procedure includes RNA extraction, fragmentation, RNA immunoprecipitation, and library construction.
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Acknowledgments
This work was supported by Ministry of Science and Technology of the People’s Republic of China (2016YFD0101001) to X.G., National Natural Science Foundation of China (31671670) to X.G., and Recruitment program of Global Youth Expert of China to X.G.
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Gu, X., Liang, Z. (2019). Transcriptome-Wide Mapping 5-Methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant. In: Chekanova, J.A., Wang, HL.V. (eds) Plant Long Non-Coding RNAs. Methods in Molecular Biology, vol 1933. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9045-0_24
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DOI: https://doi.org/10.1007/978-1-4939-9045-0_24
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9044-3
Online ISBN: 978-1-4939-9045-0
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