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Plant MicroRNAs pp 347–357Cite as

Detection of Histone Modifications Associated with miRNAs

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1932))

Abstract

The posttranslational modifications of histones and miRNAs are key epigenetic mechanisms participating in the development, growth, and reproduction of plants. Recently, coordination between these two mechanisms has been demonstrated; each mechanism can be controlled by the other for the regulation of several biological processes. For example, the acetylation of histone H3, a key modification for chromatin remodeling and gene activation, has been linked to the actions of miRNA. In this work, we describe a method for the isolation and immunodetection of two posttranslational modifications in the residues of lysine 9 and 27 of H3, which have been associated with long miRNAs in plants.

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Acknowledgments

This work was supported by grants from Consejo Nacional de Ciencia y Tecnología (CONACYT) to CD (CB2016-285898 and FC2016-1515) and a CONACYT scholarship to SHC (271240).

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Authors and Affiliations

  1. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Sara Hernández-Castellano & Clelia De-la-Peña

Authors
  1. Sara Hernández-Castellano
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  2. Clelia De-la-Peña
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Corresponding author

Correspondence to Clelia De-la-Peña .

Editor information

Editors and Affiliations

  1. Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Guanajuato, Mexico

    Stefan de Folter

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Hernández-Castellano, S., De-la-Peña, C. (2019). Detection of Histone Modifications Associated with miRNAs. In: de Folter, S. (eds) Plant MicroRNAs. Methods in Molecular Biology, vol 1932. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9042-9_25

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  • DOI: https://doi.org/10.1007/978-1-4939-9042-9_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9041-2

  • Online ISBN: 978-1-4939-9042-9

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