Abstract
A decade ago the discovery of the target mimicry regulatory process on the activity of a mature microRNA (miRNA) enabled for the first time the customized attenuation of miRNA activity in plants. That powerful technology was named MIMIC and was based on engineering the IPS1 long noncoding transcript to become complementary to the miRNA under study. In order to avoid IPS1 degradation, the predicted miRNA-mediated cleavage site was interrupted by three additional nucleotides giving rise to the so-called MIMIC decoy. Since then, MIMIC technology has been used in several plant species and in basic and translational research. We here provide a detailed guide to produce custom-designed MIMIC decoys to facilitate the study of sRNA functions in plants.
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Acknowledgments
The work in the MoRE group is funded by Spanish Ministry of Economy and Competitiveness (I.R-S is supported by BFU-2014-58361-JIN, RYC-2015-19154; L. V-M is supported by BES-2016-076986) and through the “Severo Ochoa Programme for Centres of Excellence in R&D” 2016-2019 (SEV-2015-0533) and the CERCA program from the Generalitat de Catalunya.
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Villar-Martin, L.M., Rubio-Somoza, I. (2019). Mimicry Technology: A Versatile Tool for Small RNA Suppression. In: de Folter, S. (eds) Plant MicroRNAs. Methods in Molecular Biology, vol 1932. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9042-9_18
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DOI: https://doi.org/10.1007/978-1-4939-9042-9_18
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-9042-9
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