Abstract
Tissue printing and dot blot are simple techniques to detect miRNA expression and localization, allowing a better understanding of the function of a miRNA. In this work, we describe a tissue printing and a dot blot hybridization protocol for miRNA detection and localization in plant tissues, which opens the possibility of analyzing spatiotemporal expression patterns of miRNAs.
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Acknowledgments
We thank the Mexican National Council of Science and Technology (CONACyT) for a fellowship to MMN. This work was financed by the CONACyT grant CB-2013-221522, and SIP grants 20170477 and 20180545. Work in the SDF laboratory was financed by the CONACyT grants CB-2012-177739 and FC-2015-2/1061.
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Martínez Núñez, M., de Folter, S., Rosas-Cárdenas, F.d.F. (2019). Detection of miRNAs by Tissue Printing and Dot Blot Hybridization. In: de Folter, S. (eds) Plant MicroRNAs. Methods in Molecular Biology, vol 1932. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9042-9_11
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DOI: https://doi.org/10.1007/978-1-4939-9042-9_11
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