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S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions

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Book cover Calcium-Binding Proteins of the EF-Hand Superfamily

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1929))

Abstract

Oncologic relevant members of S100 proteins are described as promising biomarkers in molecular pathology for risk estimation in oral neoplasia exhibiting different stages of malignancy: gingiva as healthy tissue, irritation fibroma as benign, leukoplakia as precancerous, and oral squamous cell carcinoma as malignant entity. Gene expression levels of S100A4 (metastasin), S100A7 (psoriasin), S100A8 (calgranulin A), and S100A9 (calgranulin B) were analyzed using quantitative RT-PCR. In addition, immunohistochemistry-based microscopy was used to examine cellular localization and distribution of these biomarkers in tissue sections. The results indicate that S100 proteins represent promising biomarkers for early-stage diagnosis in oral lesions. The inclusion of expression profiles and ratios for each entity even improves their diagnostic validity.

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Acknowledgment

R. Probstmeier and D. Kraus contributed equally to this work.

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Author notes

  1. Rainer Probstmeier and Dominik Kraus contributed equally to this work.

Authors and Affiliations

  1. Neuro- and Tumor Cell Biology Group, Department of Nuclear Medicine, University Hospital Bonn, University of Bonn, Bonn, Germany

    Rainer Probstmeier

  2. Department of Prosthodontics, Preclinical Education, and Material Sciences, University of Bonn, Bonn, Germany

    Dominik Kraus

  3. Department of Oral and Maxillofacial Plastic Surgery, University of Bonn, Bonn, Germany

    Matthias Wenghoefer

  4. Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany

    Jochen Winter

Authors
  1. Rainer Probstmeier
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  2. Dominik Kraus
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  3. Matthias Wenghoefer
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  4. Jochen Winter
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Corresponding author

Correspondence to Jochen Winter .

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Editors and Affiliations

  1. Division of Clinical Chemistry and Biochemistry, Department of Pediatrics, University of Zürich, Zürich, Switzerland

    Claus W. Heizmann

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Probstmeier, R., Kraus, D., Wenghoefer, M., Winter, J. (2019). S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions. In: Heizmann, C. (eds) Calcium-Binding Proteins of the EF-Hand Superfamily. Methods in Molecular Biology, vol 1929. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9030-6_48

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  • DOI: https://doi.org/10.1007/978-1-4939-9030-6_48

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9029-0

  • Online ISBN: 978-1-4939-9030-6

  • eBook Packages: Springer Protocols

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