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Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults

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Calcium-Binding Proteins of the EF-Hand Superfamily

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1929))

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Abstract

In recent years there have been numerous attempts to establish reliable and consistent noninvasive biomarkers of gut inflammation. Here we describe the development of an enzyme-linked immunoassay for the measurement of the pro-inflammatory, calcium-binding protein S100A12 in fecal samples.

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References

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Acknowledgment

We would like to acknowledge the contribution of Professor Carolyn Geczy as this fecal assay is based on the detection of serum S100A12 assay developed in her laboratory.

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Correspondence to Andrew S. Day .

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Leach, S.T., Day, A.S. (2019). Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults. In: Heizmann, C. (eds) Calcium-Binding Proteins of the EF-Hand Superfamily. Methods in Molecular Biology, vol 1929. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9030-6_47

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  • DOI: https://doi.org/10.1007/978-1-4939-9030-6_47

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9029-0

  • Online ISBN: 978-1-4939-9030-6

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