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Cancer Metabolism pp 45–53Cite as

Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1928))

Abstract

Radionuclide imaging with cellular-scale resolution allows characterization of biological processes and metabolic fluxes in single live cells. In this protocol, we describe how to image radiotracer uptake with single-cell resolution and compare the method to conventional bulk-scale gamma counting. We describe the utility of both techniques, give examples where each technique is recommended, and provide detailed side-by-side instructions for both techniques.

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References

  1. Sengupta D, Pratx G (2016) Single-cell characterization of FLT uptake with radioluminescence microscopy. J Nucl Med 57(7):1136–1140. https://doi.org/10.2967/jnumed.115.167734

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Author information

Authors and Affiliations

  1. Division of Medical Physics, Department of Radiation Oncology, Stanford University, Stanford, CA, USA

    Debanti Sengupta & Guillem Pratx

Authors
  1. Debanti Sengupta
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  2. Guillem Pratx
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Corresponding author

Correspondence to Guillem Pratx .

Editor information

Editors and Affiliations

  1. National Cancer Institute, North Bethesda, MD, USA

    Majda Haznadar

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Cite this protocol

Sengupta, D., Pratx, G. (2019). Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells. In: Haznadar, M. (eds) Cancer Metabolism. Methods in Molecular Biology, vol 1928. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9027-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9027-6_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9026-9

  • Online ISBN: 978-1-4939-9027-6

  • eBook Packages: Springer Protocols

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