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Recombinant Protein Production in Yeast pp 343–350Cite as

Purification of Recombinant Glycoproteins from Pichia pastoris Culture Supernatants

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1923))

Abstract

Pichia pastoris is a common host organism for the production of recombinant proteins. While unglycosylated recombinant proteins derived from this yeast can be purified efficiently by only a few conventional chromatography steps, the purification of glycosylated recombinant proteins is a very challenging process due to the intrinsic feature of the yeast of hypermannosylation. The resulting vast glycosylation pattern on the recombinant target protein masks its physicochemical properties hampering a conventional downstream process. Here, we describe a fast and efficient two-step chromatography strategy, where both steps are operated in flow-through mode, to purify recombinant glycoproteins from P. pastoris culture supernatants.

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Author information

Authors and Affiliations

  1. Institute of Chemical, Environmental and Bioscience Engineering, Research Division Biochemical Engineering, Vienna University of Technology (TU Wien), Vienna, Austria

    David Johannes Wurm & Oliver Spadiut

Authors
  1. David Johannes Wurm
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  2. Oliver Spadiut
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Corresponding author

Correspondence to Oliver Spadiut .

Editor information

Editors and Affiliations

  1. Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU) and Austrian Centre of Industrial Biotechnology (acib), Vienna, Austria

    Brigitte Gasser

  2. Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU) and Austrian Centre of Industrial Biotechnology (acib), Vienna, Austria

    Diethard Mattanovich

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Wurm, D.J., Spadiut, O. (2019). Purification of Recombinant Glycoproteins from Pichia pastoris Culture Supernatants. In: Gasser, B., Mattanovich, D. (eds) Recombinant Protein Production in Yeast. Methods in Molecular Biology, vol 1923. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9024-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9024-5_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9023-8

  • Online ISBN: 978-1-4939-9024-5

  • eBook Packages: Springer Protocols

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