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Purification of Functional F-ATP Synthase from Blue Native PAGE

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Calcium Signalling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1925))

Abstract

In the presence of Ca2+, F-ATP synthase preparations eluted from Blue Native gels generate electrophysiological currents that are typical of an inner mitochondrial membrane mega-channel, the permeability transition pore. Here we describe an experimental protocol for purification of F-ATP synthase that allows to maintain the enzyme assembly and activity that are essential for catalysis and channel formation.

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Acknowledgment

We would like to thank Paolo Bernardi (University of Padova), Giovanna Lippe (University of Udine), and Ildikò Szabò (University of Padova) for their helpful suggestions. This work was supported by AIRC grant MFAG 2017 20316 to V.G.

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Authors and Affiliations

  1. Neuroscience Institute and Department of Biomedical Sciences, CNR and University of Padua, Padua, Italy

    Chiara Galber, Giulia Valente & Valentina Giorgio

  2. Department of Biology, University of Padua, Padua, Italy

    Sophia von Stockum

  3. Fondazione Ospedale San Camillo, IRCCS, Venezia, Italy

    Sophia von Stockum

Authors
  1. Chiara Galber
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  2. Giulia Valente
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  3. Sophia von Stockum
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  4. Valentina Giorgio
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Corresponding author

Correspondence to Valentina Giorgio .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Anna Raffaello

  2. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Denis Vecellio Reane

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Galber, C., Valente, G., von Stockum, S., Giorgio, V. (2019). Purification of Functional F-ATP Synthase from Blue Native PAGE. In: Raffaello, A., Vecellio Reane, D. (eds) Calcium Signalling. Methods in Molecular Biology, vol 1925. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9018-4_20

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  • DOI: https://doi.org/10.1007/978-1-4939-9018-4_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9017-7

  • Online ISBN: 978-1-4939-9018-4

  • eBook Packages: Springer Protocols

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