Abstract
Calcium ion (Ca2+) is a ubiquitous intracellular messenger able to generate versatile intracellular signals that modulate a large variety of functions in virtually every cell type. Chemical and genetic biosensors, targeted to different subcellular compartments, have been developed and continuously improved to monitor Ca2+ dynamics in living cells. Here we describe the usage of Förster resonance energy transfer (FRET)-based Cameleon probes to investigate Ca2+ influx across the plasma membrane (PM) or Ca2+ release from the main intracellular Ca2+ store, the endoplasmic reticulum (ER).
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Acknowledgment
This work was supported by Telethon GGP16029, the University of Padua, and the EU Joint Programme for Neurodegenerative Disease Research (JPND) CeBioND, supported by the MIUR (DM 9; 08/01/2015).
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Galla, L., Pizzo, P., Greotti, E. (2019). Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling. In: Raffaello, A., Vecellio Reane, D. (eds) Calcium Signalling. Methods in Molecular Biology, vol 1925. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9018-4_2
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DOI: https://doi.org/10.1007/978-1-4939-9018-4_2
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