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Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2

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Calcium Signalling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1925))

Abstract

The store-operated calcium (Ca2+) entry (SOCE) pathway is an essential Ca2+ signaling pathway in non-excitable cells that serve many physiological functions. SOCE is mediated through the plasma membrane (PM) protein, Orai1, and the endoplasmic reticulum protein, stromal interaction molecule 1 (STIM1). One of the most well-established methods to study SOCE is using the Ca2+-sensing dye, fura-2. Here we describe a detailed protocol on how to use fura-2 to study Ca2+ signaling from SOCE in human embryonic kidney (HEK) cells.

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Acknowledgments

I would like to thank Mohamed Trebak, Ryan Yoast, and Scott Emrich for critical reading of this manuscript. Research in the author’s laboratory is supported by grants from the NIH.

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Authors and Affiliations

  1. Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA, USA

    Martin Johnson

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  1. Martin Johnson
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Correspondence to Martin Johnson .

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Editors and Affiliations

  1. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Anna Raffaello

  2. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Denis Vecellio Reane

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Johnson, M. (2019). Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2. In: Raffaello, A., Vecellio Reane, D. (eds) Calcium Signalling. Methods in Molecular Biology, vol 1925. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9018-4_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9018-4_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9017-7

  • Online ISBN: 978-1-4939-9018-4

  • eBook Packages: Springer Protocols

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