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Assays of Protonemal Growth Responses in Physcomitrella patens Under Blue- and Red-Light Stimuli

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1924))

Abstract

The non-seed land plant Physcomitrella patens is a model species for developmental, cellular, and molecular biology studies in mosses and also for performing genetic analyses. Previously, it was shown that wild-type P. patens displays a unique photomorphogenetic behavior, in which chloronemal filaments grow in the opposite direction to a blue-light source. Here, we describe bioassay systems that can be used to study light avoidance responses as well as other aspects of photomorphogenetic regulation in P. patens grown under red- and blue-light sources.

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Acknowledgments

Japan Society for the Promotion of Science (JSPS) KAKENHI grants 24380060/15H04492 to MN and HK and 16K18693 to SM; the Agricultural Chemical Research Foundation to SM; and Wada Kunko-kai Research Foundation to MN, supported this work.

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Correspondence to Hiroshi Kawaide .

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Miyazaki, S., Nakajima, M., Kawaide, H. (2019). Assays of Protonemal Growth Responses in Physcomitrella patens Under Blue- and Red-Light Stimuli. In: Yamamoto, K. (eds) Phototropism. Methods in Molecular Biology, vol 1924. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9015-3_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9015-3_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9014-6

  • Online ISBN: 978-1-4939-9015-3

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