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Vertebrate Embryogenesis pp 353–375Cite as

Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1920))

Abstract

Zebrafish (Danio rerio) is an established animal model to study developmental biology as well as a wide array of human diseases. Here we describe a protocol for somatic cell nuclear transfer (SCNT). This protocol can be used to introduce genetic modifications in zebrafish and for the study of cell plasticity.

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Author information

Authors and Affiliations

  1. Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom, Thailand

    Sukumal Prukudom & Kannika Siripattarapravat

  2. Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, Thailand

    Sukumal Prukudom & Kannika Siripattarapravat

  3. Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand

    Kannika Siripattarapravat

  4. Kasetsart University, Bangkok, Thailand

    Sukumal Prukudom & Kannika Siripattarapravat

  5. Department of Animal Science, Michigan State University, East Lansing, MI, USA

    William Poulos & Jose B. Cibelli

  6. Large Animal Clinical Sciences Department, Michigan State University, East Lansing, MI, USA

    Jose B. Cibelli

Authors
  1. Sukumal Prukudom
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  2. Kannika Siripattarapravat
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  3. William Poulos
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  4. Jose B. Cibelli
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Corresponding author

Correspondence to Jose B. Cibelli .

Editor information

Editors and Affiliations

  1. Laboratory of Genetics, University of Wisconsin–Madison, Madison, WI, USA

    Francisco J. Pelegri

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Cite this protocol

Prukudom, S., Siripattarapravat, K., Poulos, W., Cibelli, J.B. (2019). Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT). In: Pelegri, F. (eds) Vertebrate Embryogenesis. Methods in Molecular Biology, vol 1920. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9009-2_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9009-2_22

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9008-5

  • Online ISBN: 978-1-4939-9009-2

  • eBook Packages: Springer Protocols

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