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Visualizing the Balbiani Body in Zebrafish Oocytes

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Vertebrate Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1920))

Abstract

Approaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes.

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Acknowledgments

This work was partially supported by the National Institutes of Health R01GM089979.

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Authors and Affiliations

  1. Department of Cell Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    KathyAnn L. Lee & Florence L. Marlow

Authors
  1. KathyAnn L. Lee
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  2. Florence L. Marlow
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Corresponding author

Correspondence to Florence L. Marlow .

Editor information

Editors and Affiliations

  1. Laboratory of Genetics, University of Wisconsin–Madison, Madison, WI, USA

    Francisco J. Pelegri

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Lee, K.L., Marlow, F.L. (2019). Visualizing the Balbiani Body in Zebrafish Oocytes. In: Pelegri, F. (eds) Vertebrate Embryogenesis. Methods in Molecular Biology, vol 1920. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9009-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9009-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9008-5

  • Online ISBN: 978-1-4939-9009-2

  • eBook Packages: Springer Protocols

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