Abstract
The proper use of controllable atmospheric containers can facilitate investigations related to the survival abilities, and physiological states of key and emerging foodborne pathogens under recreated applicable food processing environmental conditions. Of particular note, the use of saturated salt solutions can efficiently control relative humidity in airtight containers. This chapter describes a practical experimental setup, with necessary prerequisites for exposing foodborne pathogens to simulated and relevant food processing environmental conditions. Subsequent analyses for studying cell physiology will also be suggested.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bower CK, Daeschel MA (1999) Resistance responses of microorganisms in food environments. Int J Food Microbiol 50(1–2):33–44
Carrasco E, Morales-Rueda A, Garcia-Gimeno RM (2012) Cross-contamination and recontamination by salmonella in foods: a review. Food Res Int 45(2):545–556
Todd ECD, Greig JD, Bartleson CA, Michaels BS (2009) Outbreaks where food workers have been implicated in the spread of foodborne disease. Part 6. Transmission and survival of pathogens in the food processing and preparation environment. J Food Protect 72(1):202–219
Stellato G, De Filippis F, La Storia A, Ercolini D (2015) Coexistence of lactic acid Bacteria and potential spoilage microbiota in a dairy processing environment. Appl Environ Microb 81(22):7893–7904
Ronnqvist M, Ratto M, Tuominen P, Salo S, Maunula L (2013) Swabs as a tool for monitoring the presence of Norovirus on environmental surfaces in the food industry. J Food Protect 76(8):1421–1428
Stellato G, Utter DR, Voorhis A, De Angelis M, Eren AM, Ercolini D (2017) A few Pseudomonas Oligotypes dominate in the meat and dairy processing environment. Front Microbiol 8
Habimana O, Nesse LL, Moretro T, Berg K, Heir E, Vestby LK, Langsrud S (2014) The persistence of Salmonella following desiccation under feed processing environmental conditions: a subject of relevance. Lett Appl Microbiol 59(5):464–470
Moretro T, Hoiby-Pettersen GS, Habimana O, Heir E, Langsrud S (2011) Assessment of the antibacterial activity of a triclosan-containing cutting board. Int J Food Microbiol 146(2):157–162
Moretro T, Heir E, Mo KR, Habimana O, Abdelgani A, Langsrud S (2010) Factors affecting survival of Shigatoxin-producing Escherichia coli on abiotic surfaces. Int J Food Microbiol 138(1-2):71–77
Asadishad B, Ghoshal S, Tufenkji N (2011) Method for the direct observation and quantification of survival of Bacteria attached to negatively or positively charged surfaces in an aqueous medium. Environ Sci Technol 45(19):8345–8351
Zotta T, Guidone A, Tremonte P, Parente E, Ricciardi A (2012) A comparison of fluorescent stains for the assessment of viability and metabolic activity of lactic acid bacteria. World J Microb Biot 28(3):919–927
Sunny-Roberts EO, Knorr D (2008) Evaluation of the response of lactobacillus rhamnosus VTT E-97800 to sucrose-induced osmotic stress. Food Microbiol 25(1):183–189
Zhao W, Yang RJ, Zhang HQ, Zhang WB, Hua XA, Tang YL (2011) Quantitative and real time detection of pulsed electric field induced damage on Escherichia coli cells and sublethally injured microbial cells using flow cytometry in combination with fluorescent techniques. Food Control 22(3-4):566–573
Greenspan L (1977) Humidity fixed-points of binary saturated aqueous-solutions. J Res Nbs a Phys Ch 81(1):89–96
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Habimana, O. (2019). Method to Study the Survival Abilities of Foodborne Bacterial Pathogens Under Food Processing Conditions. In: Bridier, A. (eds) Foodborne Bacterial Pathogens. Methods in Molecular Biology, vol 1918. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9000-9_8
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9000-9_8
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-8999-7
Online ISBN: 978-1-4939-9000-9
eBook Packages: Springer Protocols