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Foodborne Bacterial Pathogens pp 149–164Cite as

Identification of Putative Biomarkers Specific to Foodborne Pathogens Using Metabolomics

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1918))

Abstract

Metabolomics is one of the more recently developed “omics” that measures low molecular weight (typically < 1500 Da) compounds in biological samples. Metabolomics has been widely explored in environmental, clinical, and industrial biotechnology applications. However, its application to the area of food safety has been limited but preliminary work has demonstrated its value. This chapter describes an untargeted (nontargeted) metabolomics workflow using gas chromatography coupled to mass spectrometry (GC-MS) for characterizing three globally important foodborne pathogens, Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella enterica, from selective enrichment liquid culture media. The workflow involves a detailed description of food spiking experiments followed by procedures for extraction of polar metabolites from media, analyzing the extracts using GC-MS and, finally, chemometric data analysis using the software “SIMCA” to identify potential pathogen-specific biomarkers.

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Acknowledgments

The authors would like to thank the Australian Meat Processor Corporation (AMPC) for funding this research under the Research, Development, and Extension program 2014–2015.

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Authors and Affiliations

  1. Department of Chemistry and Biotechnology, School of Science, Swinburne University of Technology, Melbourne, VIC, Australia

    Snehal R. Jadhav, Rohan M. Shah, Avinash V. Karpe & Enzo A. Palombo

  2. Centre for Advanced Sensory Science, School of Exercise and Nutrition Sciences, Deakin University, Melbourne, VIC, Australia

    Snehal R. Jadhav

  3. Land and Water, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Brisbane, QLD, Australia

    Avinash V. Karpe & David J. Beale

  4. Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, Australia

    Konstantinos A. Kouremenos

Authors
  1. Snehal R. Jadhav
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  2. Rohan M. Shah
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  3. Avinash V. Karpe
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  4. David J. Beale
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  5. Konstantinos A. Kouremenos
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  6. Enzo A. Palombo
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Corresponding author

Correspondence to Enzo A. Palombo .

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Editors and Affiliations

  1. Fougères Laboratory, ANSES, Fougères, France

    Arnaud Bridier

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Jadhav, S.R., Shah, R.M., Karpe, A.V., Beale, D.J., Kouremenos, K.A., Palombo, E.A. (2019). Identification of Putative Biomarkers Specific to Foodborne Pathogens Using Metabolomics. In: Bridier, A. (eds) Foodborne Bacterial Pathogens. Methods in Molecular Biology, vol 1918. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9000-9_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9000-9_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8999-7

  • Online ISBN: 978-1-4939-9000-9

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