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Bone Research Protocols pp 641–649Cite as

Fourier Transform Infrared Imaging of Bone

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

Abstract

Fourier transform infrared imaging (FTIRI) is a technique that can be used to analyze the material properties of bone using tissue sections. This chapter describes the basic principles of FTIR and the methods for capturing and analyzing FTIR images in bone sections.

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References

  1. Marcott C, Reeder RC, Paschalis EP, Tatakis DN, Boskey AL, Mendelsohn R (1998) Infrared microspectroscopic imaging of biomineralized tissues using a mercury-cadmium-telluride focal-plane array detector. Cell Mol Biol (Noisy-le-Grand) 44(1):109–115 Epub 1998/04/29

    CAS  Google Scholar 

  2. Paschalis EP, Verdelis K, Doty SB, Boskey AL, Mendelsohn R, Yamauchi M (2001) Spectroscopic characterization of collagen cross-links in bone. J Bone Miner Res 16(10):1821–1828 Epub 2001/10/05

    Article  CAS  Google Scholar 

  3. Camacho NP, Rinnerthaler S, Paschalis EP, Mendelsohn R, Boskey AL, Fratzl P (1999) Complementary information on bone ultrastructure from scanning small angle X-ray scattering and Fourier-transform infrared microspectroscopy. Bone 25(3):287–293 Epub 1999/09/24

    Article  CAS  Google Scholar 

  4. Paschalis EP, Tatakis DN, Robins S, Fratzl P, Manjubala I, Zoehrer R et al (Dec 2011) Lathyrism-induced alterations in collagen cross-links influence the mechanical properties of bone material without affecting the mineral. Bone 49(6):1232–1241

    Article  CAS  Google Scholar 

  5. Durchschlag E, Paschalis EP, Zoehrer R, Roschger P, Fratzl P, Recker R et al (2006) Bone material properties in trabecular bone from human iliac crest biopsies after 3- and 5-year treatment with risedronate. J Bone Miner Res 21(10):1581–1590 Epub 2006/09/26

    Article  CAS  Google Scholar 

  6. Boskey A, Mendelsohn R (2005) Infrared analysis of bone in health and disease. J Biomed Opt 10(3):031102 Epub 2005/10/19

    Article  Google Scholar 

  7. Boskey A, Pleshko Camacho N (2007) FT-IR imaging of native and tissue-engineered bone and cartilage. Biomaterials 28(15):2465–2478 Epub 2006/12/19

    Article  CAS  Google Scholar 

  8. Gourion-Arsiquaud S, West PA, Boskey AL (2008) Fourier transform-infrared microspectroscopy and microscopic imaging. Methods Mol Biol 455:293–303 Epub 2008/05/09

    Article  CAS  Google Scholar 

  9. Paschalis EP (2009) Fourier transform infrared analysis and bone. Osteoporos Int 20(6):1043–1047 Epub 2009/04/03

    Article  CAS  Google Scholar 

  10. Gamsjaeger S, Mendelsohn R, Boskey AL, Gourion-Arsiquaud S, Klaushofer K, Paschalis EP (2014) Vibrational spectroscopic imaging for the evaluation of matrix and mineral chemistry. Curr Osteoporos Rep 12(4):454–464

    Article  CAS  Google Scholar 

  11. Misof BM, Gamsjaeger S, Cohen A, Hofstetter B, Roschger P, Stein E et al (2012) Bone material properties in premenopausal women with idiopathic osteoporosis. J Bone Miner Res 27(12):2551–2561

    Article  CAS  Google Scholar 

  12. Paschalis EP, Gamsjaeger S, Klaushofer K (2017) Vibrational spectroscopic techniques to assess bone quality. Osteoporos Int 28(8):2275–2291

    Article  CAS  Google Scholar 

  13. Paschalis EP, Shane E, Lyritis G, Skarantavos G, Mendelsohn R, Boskey AL (2004) Bone fragility and collagen cross-links. J Bone Miner Res 19(12):2000–2004 Epub 2004/11/13

    Article  Google Scholar 

  14. Boskey AL, DiCarlo E, Paschalis E, West P, Mendelsohn R (2005) Comparison of mineral quality and quantity in iliac crest biopsies from high- and low-turnover osteoporosis: an FT-IR microspectroscopic investigation. Osteoporos Int 16(12):2031–2038 Epub 2005/08/10

    Article  CAS  Google Scholar 

  15. Paschalis EP, Betts F, DiCarlo E, Mendelsohn R, Boskey AL (1997) FTIR microspectroscopic analysis of human iliac crest biopsies from untreated osteoporotic bone. Calcif Tissue Int 61(6):487–492 Epub 1998/02/12

    Article  CAS  Google Scholar 

  16. Paschalis EP, DiCarlo E, Betts F, Sherman P, Mendelsohn R, Boskey AL (1996) FTIR microspectroscopic analysis of human osteonal bone. Calcif Tissue Int 59(6):480–487 Epub 1996/12/01

    Article  CAS  Google Scholar 

  17. Ou-Yang H, Paschalis EP, Mayo WE, Boskey AL, Mendelsohn R (2001) Infrared microscopic imaging of bone: spatial distribution of CO3(2−). J Bone Miner Res 16(5):893–900 Epub 2001/05/09

    Article  CAS  Google Scholar 

  18. Paschalis EP, Gamsjaeger S, Tatakis DN, Hassler N, Robins SP, Klaushofer K (2015) Fourier transform infrared spectroscopic characterization of mineralizing type I collagen enzymatic trivalent cross-links. Calcif Tissue Int 96(1):18–29

    Article  CAS  Google Scholar 

  19. Aparicio S, Doty SB, Camacho NP, Paschalis EP, Spevak L, Mendelsohn R et al (2002) Optimal methods for processing mineralized tissues for Fourier transform infrared microspectroscopy. Calcif Tissue Int 70(5):422–429 Epub 2002/06/11

    Article  CAS  Google Scholar 

  20. Blouin S, Thaler HW, Korninger C, Schmid R, Hofstaetter JG, Zoehrer R et al (2009) Bone matrix quality and plasma homocysteine levels. Bone 44(5):959–964 Epub 2009/01/27

    Article  CAS  Google Scholar 

  21. Paschalis EP, Boskey AL, Kassem M, Eriksen EF (2003) Effect of hormone replacement therapy on bone quality in early postmenopausal women. J Bone Miner Res 18(6):955–959 Epub 2003/06/24

    Article  CAS  Google Scholar 

  22. Paschalis EP, Glass EV, Donley DW, Eriksen EF (2005) Bone mineral and collagen quality in iliac crest biopsies of patients given teriparatide: new results from the fracture prevention trial. J Clin Endocrinol Metab 90(8):4644–4649 Epub 2005/05/26

    Article  CAS  Google Scholar 

  23. Paschalis EP, Burr DB, Mendelsohn R, Hock JM, Boskey AL (2003) Bone mineral and collagen quality in humeri of ovariectomized cynomolgus monkeys given rhPTH(1-34) for 18 months. J Bone Miner Res 18(4):769–775 Epub 2003/04/04

    Article  CAS  Google Scholar 

  24. Paschalis EP, Recker R, DiCarlo E, Doty SB, Atti E, Boskey AL (2003) Distribution of collagen cross-links in normal human trabecular bone. J Bone Miner Res 18(11):1942–1946 Epub 2003/11/11

    Article  CAS  Google Scholar 

  25. Roschger P, Manjubala I, Zoeger N, Meirer F, Simon R, Li C et al (2009) Bone material quality in Transiliac bone biopsies of postmenopausal osteoporotic women after 3 years of strontium Ranelate treatment. J Bone Miner Res. 2010 Apr;25(4):891–900

    Google Scholar 

  26. Gourion-Arsiquaud S, Faibish D, Myers E, Spevak L, Compston J, Hodsman A et al (2009) Use of FTIR spectroscopic imaging to identify parameters associated with fragility fracture. J Bone Miner Res 24(9):1565–1571 Epub 2009/05/08

    Article  Google Scholar 

  27. Lieber CA, Mahadevan-Jansen A (2003) Automated method for subtraction of fluorescence from biological Raman spectra. Appl Spectrosc 57:1363–1367

    Article  CAS  Google Scholar 

  28. Dong A, Huang P, Caughey WS (1990) Protein secondary structures in water from second-derivative amide I infrared spectra. Biochemistry 29:3303–3308

    Article  CAS  Google Scholar 

  29. Mendelsohn R, Paschalis EP, Boskey AL (1999) Infrared spectroscopy, microscopy, and microscopic imaging of mineralizing tissues. Spectra-structure correlations from human iliac crest biopsies. J Biomed Opt 4(1):14–21

    Article  CAS  Google Scholar 

  30. Mendelsohn R, Paschalis EP, Sherman PJ, Boskey AL (2000) IR microscopic imaging of pathological states and fracture healing of bone. Appl Spectrosc 54:1183–1191

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Ludwig Boltzmann Institute for Osteology, Hanusch Krankenhaus, Vienna, Austria

    Eleftherios P. Paschalis

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  1. Eleftherios P. Paschalis
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Correspondence to Eleftherios P. Paschalis .

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

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Paschalis, E.P. (2019). Fourier Transform Infrared Imaging of Bone. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_34

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  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_34

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

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