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Mouse Models of Melanoma Bone Metastasis

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

Abstract

Melanomas are aggressive cancers of the skin with high metastatic capacity. Mouse models are necessary to delineate the mechanisms of cancer metastasis and xenograft models can also allow examining the role of the host using different genetically-modified mouse models. In this chapter, I provide a detailed protocol for the preparation and inoculation of tumor cells intra-cardially and intra-tibially to achieve bone metastasis.

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References

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Acknowledgments

I would like to acknowledge Dr. Katherine Weilbaecher, whose lab I learned all these techniques in during my graduate studies. Specific thanks go to my fellow labmates Dr. Angela Hirbe and Dr. Elizabeth Morgan.

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Authors and Affiliations

  1. Novartis Institutes for Biomedical Research, Basel, Switzerland

    Özge Uluçkan

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  1. Özge Uluçkan
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Correspondence to Özge Uluçkan .

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Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

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Uluçkan, Ö. (2019). Mouse Models of Melanoma Bone Metastasis. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_19

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  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

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