Abstract
Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice breeding system under both long-day and short-day conditions. The isolation and backcross process used for this could easily last for more than 3 years with a very low success rate. Here, we describe a straightforward method for generating csa-based PGMS lines by using the CRISPR-Cas9 technology in rice.
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References
Cheng SH, Zhuang JY, Fan YY, Du JH, Cao LY (2007) Progress in research and development on hybrid rice: a super-domesticate in China. Ann Bot 100:959–966
Mou T, Cao B, Huang Z, Yang G, Lin Y, Xiang G (2001) Characterization of fertility alteration in 8 environment-sensitive genic male-sterile lines of indica rice. Acta Bot Sin 43:238–242
Zhang HL, Chen XY, Huang JZ, Zhi Guo E, Gong JY, Shu QY (2015) Identification and transition analysis of photo-/thermo-sensitive genic male sterile genes in two-line hybrid rice in China. Sci Agric Sin 31:175–182
Woo M, Ham T, Ji H, Choi M, Jiang W, Chu S, Piao R, Chin J, Kim J, Park B (2008) Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.). Plant J 54:190
Li Q, Zhang D, Chen M, Liang W, Wei J, Qi Y, Yuan Z (2016) Development of japonica photo-sensitive genic male sterile rice lines by editing carbon starved anther using CRISPR/Cas9. J Genet Genomics 43:415–419
Zhang H, Liang W, Yang X, Luo X, Jiang N, Ma H, Zhang D (2010) Carbon starved anther encodes a MYB domain protein that regulates sugar partitioning required for rice pollen development. Plant Cell 22:672
Zhang H, Xu C, He Y, Zong J, Yang X, Si H, Sun Z, Hu J, Liang W, Zhang D (2013) Mutation in CSA creates a new photoperiod-sensitive genic male sterile line applicable for hybrid rice seed production. Proc Natl Acad Sci U S A 110:76–81
Zhang H, Zhang J, Wei P, Zhang B, Gou F, Feng Z, Mao Y, Yang L, Zhang H, Xu N, Zhu JK (2014) The crispr/cas9 system produces specific and homozygous targeted gene editing in rice in one generation. Plant Biotechnology Journal, 12, 797–807.
Mao Y, Zhang H, Xu N, Zhang B, Gou F, Zhu JK (2013) Application of the CRISPR–Cas system for efficient genome engineering in plants. Mol Plant 6:2008
Nishimura A, Aichi I, Matsuoka M (2006) A protocol for Agrobacterium-mediated transformation in rice. Nat Protoc 1:2796–2802
Ozawa K (2012) A high-efficiency Agrobacterium-mediated transformation system of rice (Oryza sativa L.). Methods Mol Biol 847:51–57
Zhang DB, Wilson ZA (2009) Stamen specification and anther development in rice. Chin Sci Bull 54:2342–2353
Acknowledgments
This work was supported by the funds from the National Transgenic Major Program (2016ZX08009003-003-007) and National Key Research and Development Program of China (2016YFD0101107) to ZY.
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Gu, W., Zhang, D., Qi, Y., Yuan, Z. (2019). Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR/Cas9. In: Qi, Y. (eds) Plant Genome Editing with CRISPR Systems. Methods in Molecular Biology, vol 1917. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8991-1_8
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DOI: https://doi.org/10.1007/978-1-4939-8991-1_8
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