Abstract
Immunohistochemistry (IHC) is a commonly used technique for protein detection in tissue sections. The method requires high-affinity antibodies that are specific for the target proteins of interest. More advanced IHC techniques have been developed to meet the need for simultaneous detection of more than one target protein in the same tissue section. This chapter provides general guidelines for double IHC staining of formalin-fixed, paraffin-embedded tissue sections. Chromogenic substrates are chosen based on their excellent contrast and compatibility with the subsequent digital image analysis.
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Acknowledgments
This work was supported by the Svenska Institutet to AM, the Swedish Childhood Cancer Fund (Barncancerfonden) and the Tornspiran Foundation (Stiftelsen Tornspiran) to ME, and the Knut and Alice Wallenberg Foundation to PM.
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Moreno-Ruiz, P., Wik Leiss, L., Mezheyeuski, A., Ehnman, M. (2019). Double Immunohistochemistry and Digital Image Analysis. In: Pico de Coaña, Y. (eds) Immune Checkpoint Blockade. Methods in Molecular Biology, vol 1913. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8979-9_1
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DOI: https://doi.org/10.1007/978-1-4939-8979-9_1
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