Abstract
Mutagenesis is one of the key techniques in virus research. The recent development of deep mutational scanning allows the assessment of replication fitness effects of a large number of viral mutants in a high-throughput manner. Here, we describe a protocol for studying hepatitis C virus (HCV) using deep mutational scanning, which includes the methodologies for mutant library construction, passaging, sequencing, and data analysis.
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References
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Acknowledgment
NCW was supported by the Croucher Foundation Fellowship.
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Wu, N.C., Qi, H. (2019). Application of Deep Mutational Scanning in Hepatitis C Virus. In: Law, M. (eds) Hepatitis C Virus Protocols . Methods in Molecular Biology, vol 1911. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8976-8_12
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DOI: https://doi.org/10.1007/978-1-4939-8976-8_12
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8976-8
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