Abstract
Incorporation of bile drainage system into engineered liver tissue is an important issue to advance liver regenerative medicine. Our group reported that three-dimensional (3D) coculture of fetal liver cells (FLCs) and adult rat biliary epithelial cells (BECs) allows reconstruction of hepatic spheroids that possess bile ductular structures. In this chapter, we describe the detailed protocol to isolate FLCs and BECs and to generate the spheroids with bile drainage system using these two types of primary cells.
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Acknowledgments
This work was supported in part by Grant-in-Aid for Young Scientists B (16K16643) and the Research Program on Hepatitis (16fk0310505h0005) from the Japan Agency for Medical Research and Development (AMED).
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Katsuda, T., Ochiya, T., Sakai, Y. (2019). Generation of Hepatic Organoids with Biliary Structures. In: Tanimizu, N. (eds) Hepatic Stem Cells . Methods in Molecular Biology, vol 1905. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8961-4_16
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DOI: https://doi.org/10.1007/978-1-4939-8961-4_16
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8961-4
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