Abstract
Mouse fetal liver includes abundant hepatic stem/progenitor cells (HSPCs). Easy expansion with passage of HSPCs is necessary to obtain steady data. However, it is often difficult to enrich only HSPCs, and HSPCs can die when usual trypsin is used for replating. Here, we introduce serum-free long-term culture with passage of HSPCs using fetal mouse liver without a cell sorter.
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Tsuchiya, A., Terai, S. (2019). Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells. In: Tanimizu, N. (eds) Hepatic Stem Cells . Methods in Molecular Biology, vol 1905. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8961-4_1
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DOI: https://doi.org/10.1007/978-1-4939-8961-4_1
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8960-7
Online ISBN: 978-1-4939-8961-4
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