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Human Monoclonal Antibodies pp 319–338Cite as

Phage Display Technology for Human Monoclonal Antibodies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1904))

Abstract

During the last 20 years in vitro technologies opened powerful routes to combine the generation of large libraries together with fast selection and screening procedures to identify lead candidates. One of the most successful methods is based on the use of filamentous phages. Functional Antibodies (Abs) fragments can be displayed on the surface of phages by fusing the coding sequence of the antibody variable (V) regions to the phage minor coat protein pIII. By creating large libraries, antibodies with affinities comparable to those obtained using traditional hybridoma technology can be isolated by a series of cycles of selection on the antigen of interest. In this system, antibody genes can be recovered simultaneously with selection and can be easily further engineered, for example by increasing their affinity to levels unobtainable in the immune system, or by modulating their specificity and their effector functions (by recloning into a full-length immunoglobulin scaffold). This chapter describes the basic protocols for antibody library construction and selection of binder with desired specificity.

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Authors and Affiliations

  1. Department of Life Sciences, University of Trieste, Trieste, Italy

    Marco Dal Ferro, Serena Rizzo, Emanuela Rizzo, Francesca Marano, Immacolata Luisi & Daniele Sblattero

  2. Department of Health Sciences and IRCAD, University of Eastern Piedmont, Novara, Italy

    Olga Tarasiuk

Authors
  1. Marco Dal Ferro
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  2. Serena Rizzo
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  3. Emanuela Rizzo
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  4. Francesca Marano
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  5. Immacolata Luisi
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  6. Olga Tarasiuk
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  7. Daniele Sblattero
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Corresponding author

Correspondence to Daniele Sblattero .

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Editors and Affiliations

  1. Department of Pathology, The Hebrew University, Hadassah Medical School, Jerusalem, Israel

    Michael Steinitz

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Dal Ferro, M. et al. (2019). Phage Display Technology for Human Monoclonal Antibodies. In: Steinitz, M. (eds) Human Monoclonal Antibodies. Methods in Molecular Biology, vol 1904. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8958-4_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8958-4_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8957-7

  • Online ISBN: 978-1-4939-8958-4

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