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Detection of Autoantibodies to Complement Components by Surface Plasmon Resonance-Based Technology

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Autoantibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1901))

Abstract

The innate immune complement system is a powerful defense cascade against pathogens, but can induce host tissue damage when overactivated. In pathological conditions, mainly but not restricted to renal diseases, such as lupus nephritis, atypical hemolytic uremic syndrome, and C3 glomerulopathies, complement is overactivated or dysregulated by autoantibodies directed against its components and regulators. Among the key autoantibody targets are the initiator of the classical complement pathway C1q, the alternative pathway regulator Factor H, the components of the alternative pathway C3 convertase complex C3 and Factor B and the convertase complex itself. This methodological article describes our experience with a method for detection of anti-complement autoantibodies in real time using surface plasmon resonance-based technology. It allows label-free evaluation of the binding efficacy and stability of the formed antigen–antibody complexes.

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Acknowledgments

This work was supported by grants from Agence Nationale de la Recherche ANR JCJC - INFLACOMP 2015-2018 ANR-15-CE15-0001 to LTR, RILA grant to MADD (Campus France 36666QH) and MR (№DNTS/France 01/11), ANR COMPC3 ANR-16-CE18-0015 to VFB, EU FP7 grant 2012-305608 (EURenOmics) to VFB, APHP-PHRC AOM08198 to VFB, and by INSERM.

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Authors and Affiliations

  1. INSERM, UMR_S 1138, Centre de Recherche des Cordeliers, Paris, France

    Remi Noe, Sophie Chauvet, Shambhuprasad K. Togarsimalemath, Maria Chiara Marinozzi, Veronique Fremeaux-Bacchi, Marie-Agnes Dragon-Durey & Lubka T. Roumenina

  2. Sorbonne Universités, UPMC Univ Paris 06, Paris, France

    Remi Noe, Sophie Chauvet, Shambhuprasad K. Togarsimalemath, Maria Chiara Marinozzi, Veronique Fremeaux-Bacchi, Marie-Agnes Dragon-Durey & Lubka T. Roumenina

  3. Université Paris Descartes, Sorbonne Paris Cité, Paris, France

    Remi Noe, Sophie Chauvet, Shambhuprasad K. Togarsimalemath, Maria Chiara Marinozzi, Veronique Fremeaux-Bacchi, Marie-Agnes Dragon-Durey & Lubka T. Roumenina

  4. Assistance Publique–Hôpitaux de Paris, Service de néphrologie, Hôpital Européen Georges Pompidou, Paris, France

    Sophie Chauvet

  5. Department of Biochemistry, Molecular Medicine and Nutrigenomics, Medical University of Varna, Varna, Bulgaria

    Maria Radanova

  6. Nephrology Clinic, University Hospital ‘Tsaritsa Yoanna-ISUL,’ Medical University, Sofia, Bulgaria

    Vasil V. Vasilev

  7. Assistance Publique–Hôpitaux de Paris, Service d’Immunologie Biologique, Hôpital Européen Georges Pompidou, Paris, France

    Veronique Fremeaux-Bacchi & Marie-Agnes Dragon-Durey

Authors
  1. Remi Noe
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  2. Sophie Chauvet
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  3. Shambhuprasad K. Togarsimalemath
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  4. Maria Chiara Marinozzi
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  5. Maria Radanova
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  6. Vasil V. Vasilev
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  7. Veronique Fremeaux-Bacchi
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  8. Marie-Agnes Dragon-Durey
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  9. Lubka T. Roumenina
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Corresponding author

Correspondence to Lubka T. Roumenina .

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Editors and Affiliations

  1. Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

    Gunnar Houen

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Noe, R. et al. (2019). Detection of Autoantibodies to Complement Components by Surface Plasmon Resonance-Based Technology. In: Houen, G. (eds) Autoantibodies. Methods in Molecular Biology, vol 1901. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8949-2_24

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  • DOI: https://doi.org/10.1007/978-1-4939-8949-2_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8948-5

  • Online ISBN: 978-1-4939-8949-2

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