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Autoantibodies pp 243–253Cite as

Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1901))

Abstract

Anti-citrullinated protein antibodies (ACPA)s are a hallmark of rheumatoid arthritis (RA) and are essential for serological diagnosis of RA.

ACPAs are not specific for a single citrullinated target; in fact, several citrullinated ACPA target proteins have been described. As a consequence, ACPAs are primarily detected by enzyme-linked immunosorbent assays, where several citrullinated peptides are used as target antigens.

This chapter focuses on the detection of ACPAs using a recently developed peptide panel in enzyme-linked immunosorbent assays.

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  1. Department of Autoimmunology and Biomarkers, Statens Serum Institut, Copenhagen S, Denmark

    Nicole Hartwig Trier

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Correspondence to Nicole Hartwig Trier .

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  1. Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

    Gunnar Houen

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Trier, N.H. (2019). Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay. In: Houen, G. (eds) Autoantibodies. Methods in Molecular Biology, vol 1901. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8949-2_21

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  • DOI: https://doi.org/10.1007/978-1-4939-8949-2_21

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