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Immunological Tolerance pp 55–66Cite as

Identification and Isolation of Regulatory B Cells in Mouse and Human

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1899))

Abstract

Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.

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Acknowledgments

We would like to thank Dr. Paul Blair for his constructive criticism of the manuscript. Madhvi Menon is funded by a Wellcome Trust PhD studentship (090406/Z/09/Z). Elizabeth C. Rosser was funded by an Arthritis Research UK PhD studentship (MP/19314).

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Author notes

  1. Madhvi Menon and Elizabeth C. Rosser contributed equally to this work.

Authors and Affiliations

  1. Centre for Rheumatology, Division of Medicine, University College London, 5 University Street, London, WC1E 6JF, UK

    Madhvi Menon, Elizabeth C. Rosser & Claudia Mauri

Authors
  1. Madhvi Menon
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  2. Elizabeth C. Rosser
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  3. Claudia Mauri
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Corresponding author

Correspondence to Claudia Mauri .

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Editors and Affiliations

  1. Associate Professor in Stem Cell Biology and Regenerative Medicine, Research Department of Surgical Biotechnology, Division of Surgery and Interventional Science, University College London (UCL), Royal Free Hospital, London, UK

    Ashleigh S. Boyd

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Menon, M., Rosser, E.C., Mauri, C. (2019). Identification and Isolation of Regulatory B Cells in Mouse and Human. In: Boyd, A. (eds) Immunological Tolerance. Methods in Molecular Biology, vol 1899. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8938-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-8938-6_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8936-2

  • Online ISBN: 978-1-4939-8938-6

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