Abstract
Patient tumor tissue processing is an important step in the generation of clinically relevant specimens for in vitro and in vivo studies. Proper disassociation and tissue sample cleanup is a multistep, time-consuming process that ultimately effects the generation of patient derived xenografts and neurosphere cultures. Here we describe a detailed protocol on how to process and disassociate patient glioma tissue and subsequent steps on orthotopic implantation and in vitro generation of neurospheres.
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Gosa, L., Ta, L., Nathanson, D.A. (2019). Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines. In: Yong, W. (eds) Biobanking. Methods in Molecular Biology, vol 1897. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8935-5_34
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DOI: https://doi.org/10.1007/978-1-4939-8935-5_34
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8933-1
Online ISBN: 978-1-4939-8935-5
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