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Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens

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Biobanking

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1897))

Abstract

Saliva and oral/buccal samples have become increasingly valuable sources of genetic material for clinical applications. The DNA obtained by these samples is of comparable quality to that from blood samples. This, coupled with the ease of collecting saliva and oral/buccal samples, has led to increased numbers of such samples being incorporated into biobanks. This chapter will detail the steps involved in procuring, transporting, and storing saliva, buccal swabs, and oral wash samples for further use in downstream applications.

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References

  1. Pramanik R, Thompson H, Kistler JO et al (2012) Effects of the UK Biobank collection protocol on potential biomarkers in saliva. Int J Epidemiol 41:1786–1797

    Article  Google Scholar 

  2. Koni AC, Scott RA, Wang G et al (2011) DNA yield and quality of saliva samples and suitability for large-scale epidemiological studies in children. Int J Obes (Lond) 35:S113–S118

    Article  CAS  Google Scholar 

  3. Rogers NL, Cole SA, Lan H et al (2007) New saliva DNA collection method compared to buccal cell collection techniques for epidemiological studies. Am J Hum Biol 19:319–326

    Article  Google Scholar 

  4. Garcia-Closas M, Egan KM, Abruzzo J et al (2001) Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwash. Cancer Epidemiol Biomark Prev 10:687–696

    CAS  Google Scholar 

  5. Floriano PN, Christodoulides N, Miller CS et al (2009) Use of saliva-based nano-biochip tests for acute myocardial infarction at the point of care: a feasibility study. Clin Chem 55:1530–1538

    Article  CAS  Google Scholar 

  6. Hashida S, Hashinaka K, Ishikawa S et al (1997) More reliable diagnosis of infection with human immunodeficiency virus type 1 (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva and/or serum with highly sensitive and specific enzyme immunoassay (immune complex transfer enzyme immunoassay): a review. J Clin Lab Anal 11:267–286

    Article  CAS  Google Scholar 

  7. Janardhanam SB, Zunt SL, Srinivasan M (2012) Quality assessment of saliva bank samples. Biopreserv Biobank 10(3):282–287

    Article  CAS  Google Scholar 

  8. Guerin JS, Murray DW, McGrath MM et al (2010) Molecular medicine Ireland for standardised biobanking, biopreservation and biobanking March 2010 8(1):3–63. https://doi.org/10.1089/bio.2010.8101

  9. DNA Genotek (2015) Laboratory protocol for manual purification of DNA from whole sample. Available via DNA Genotek. http://www.dnagenotek.com/US/pdf/PD-PR-015.pdf. Accessed 14 Oct 2015

  10. DNA Genotek (2015) Laboratory protocol for manual purification of DNA from 0.5 mL of sample. Available via DNA Genotek. http://www.dnagenotek.com/US/pdf/PD-PR-006.pdf. Accessed 14 Oct 2015

  11. Qiagen (2010) QIAGEN supplementary protocol: isolation of genomic DNA from saliva and mouthwash using the QIAamp® DNA Blood Mini Kit; spin procedure. https://www.qiagen.com/resources/download.aspx?id=efb83e1e-76e6-417a-b479-d57eb77e0c4f&lang=en. Accessed 14 Oct 2015

  12. Qiagen (2012) QIAamp® DNA Mini and Blood Mini handbook, 3rd edn. https://www.qiagen.com/us/resources/resourcedetail?id=67893a91-946f-49b5-8033-394fa5d752ea&lang=en. Accessed 14 Oct 2015

  13. G-Biosciences (2012) XITâ„¢ Genomic DNA from buccal cells: for extraction of genomic DNA from buccal/cheek cells. Available via G-Biosciences. http://www.gbiosciences.com/PDF/Protocol/XIT_Genomic_DNA_from_Buccal_Cell.pdf. Accessed 14 Oct 2015

  14. 5 Prime: Life Science Products: PerfectPure DNA Cultured Cell Kit: DNA purification from buccal cells in mouthwash. https://www.funakoshi.co.jp/download/catalog/FPR3911.pdf. Accessed 14 Oct 2015

  15. Koppikar P, Mulherkar R (2006) A simple method for extraction of high molecular weight genomic DNA from buccal cells in mouthwash. Indian J Biotechnol 5:477–481

    CAS  Google Scholar 

  16. Le Marchand L, Lum-Jones A, Saltzman B et al (2001) Feasibility of collecting buccal DNA by mail in a cohort study. Cancer Epidemiol Biomark Prev 10:701–703

    Google Scholar 

  17. Kuchler EC, Tannure PN, Falagan-Lotsch P et al (2012) Buccal cells DNA extraction to obtain high quality human genomic DNA suitable for polymorphism genotyping by PCR-RFLP and Real-Time PCR. J Appl Oral Sci 20:467–471

    Article  CAS  Google Scholar 

  18. Heath EM, Morken NW, Campbell KA et al (2001) Use of buccal cells collected in mouthwash as a source of DNA for clinical testing. Arch Pathol Lab Med 125(1):127–133

    CAS  PubMed  Google Scholar 

  19. The UK Biobank (2011) Saliva sample collection, processing and transport. Available via Biobank UK. https://biobank.ctsu.ox.ac.uk/crystal/docs/Salivasample.pdf. Accessed 14 Oct 2015

  20. DNA Genotek (2011) Maximizing DNA yield with the Oragene® self-collection kit. Available via DNA Genotek. http://www.dnagenotek.com/US/pdf/PD-PR-010.pdf. Accessed 20 Oct 2015

  21. DNA Genotek (2011) Long-term storage of Oragene®/saliva samples. Available via DNA Genotek. http://www.dnagenotek.com/US/pdf/PD-PR-012.pdf. Accessed 20 Oct 2015

  22. Husebekk A, Iversen O-J, Langmark F et al (2003) Biobanks for health: optimising the use of European biobanks and health registries for research relevant to public health and combating disease. Report and recommendations from an EU workshop held at Voksenåsen Hotel, Oslo 28–31 January 2003. http://www.fhi.no/dav/1f1c30ab2c.pdf. Accessed 15 Oct 2015

  23. Qiagen (2010) QIAamp® 96 DNA Swab BioRobot® Kit handbook, 4th edn. https://www.qiagen.com/us/shop/sample-technologies/dna-sample-technologies/genomic-dna/qiaamp-96-dna-swab-biorobot-kit/#resources. Accessed 14 Oct 2015

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Correspondence to David Y. Lu .

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Woo, J.S., Lu, D.Y. (2019). Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens. In: Yong, W. (eds) Biobanking. Methods in Molecular Biology, vol 1897. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8935-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-8935-5_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8933-1

  • Online ISBN: 978-1-4939-8935-5

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