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Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development

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The Hippo Pathway

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1893))

Abstract

The HIPPO signaling pathway plays an early and essential role in mammalian embryogenesis. The earliest known roles for HIPPO signaling during mouse development include segregating fetal and extraembryonic lineages and establishing the pluripotent progenitors of embryonic stem (ES) cells. In the mouse early embryo, HIPPO signaling responds to multiple cell biological inputs, including cell polarization, cytoskeleton, and cell environment, to influence gene expression and the first cell fate decisions in development. Methods to monitor and manipulate HIPPO signaling in the mouse early embryo are fundamental to discovering mechanisms regulating pluripotency in vivo, but properties of the early embryo, such as small cell number and spherical architecture, pose unique challenges for signaling pathway analysis. Here, we share approaches for visualizing HIPPO signaling in mouse early embryos. In addition, these methods can be applied to visualize HIPPO signaling in other spherical or cystic structures comprised of relatively few cells, such as organoids, or for the examination of other signaling pathways in these contexts.

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Acknowledgments

Work in our lab is supported by National Institutes of Health grant R01 GM104009 and the James K. Billman, Jr., M.D. Endowment Fund.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA

    Tristan Frum & Amy Ralston

Authors
  1. Tristan Frum
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  2. Amy Ralston
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Corresponding author

Correspondence to Amy Ralston .

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Editors and Affiliations

  1. Cancer Institute, University College London, London, UK

    Alexander Hergovich

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Frum, T., Ralston, A. (2019). Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_25

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  • DOI: https://doi.org/10.1007/978-1-4939-8910-2_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8909-6

  • Online ISBN: 978-1-4939-8910-2

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